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Comparison between HPTLC / fluorometry and HPLC for the determination of sennosides A and B in Senna (Cassia spp.) pods and leaves
Summary
Type:
HPLC
Application #:
102600
Detection:
UV, 275nm
Conditions:
eluent: methanol - water - acetic acid (80:20:6.5)
flow rate 1ml/min
column temperature 45°C
Authors:
Duez R
Source:
J Chromatogr 303 (1984) 391 - 395
Details
Legend: Fig. 1. HPLC chromatogram of a Cassia italica extract, I. sennoside A, II. sennoside B.
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